![]() Being a relatively cheap and quick experiment, the HSQC is useful to screen candidates for structure determination by NMR. If the protein is folded, the peaks are usually well dispersed, and most of the individual peaks can be distinguished. The latter can be recorded on much lower concentrations of protein, but requires recombinant expression of the protein.Įach residue of the protein (except proline) has an amide proton attached to a nitrogen in the peptide bond. ![]() ![]() The HSQC experiment can be performed either using the natural abundance of the 15N isotope, or using isotopically labeled protein. The 15N HSQC experiment is probably the most frequently recorded experiment in protein NMR. Thus, if the chemical shift of a specific proton is known, the chemical shift of the coupled heteronucleus can be determined, and vice versa. The spectrum contains a peak for each unique proton attached to the heteronucleus being considered. The resulting spectrum is two-dimensional with one axis for 1H and the other for a heteronucleus (an atomic nucleus other than a proton), most often 13C or 15N. It was invented by Geoffrey Bodenhausen and D. The Heteronuclear Single Quantum Coherence (HSQC) experiment is used frequently in NMR spectroscopy of organic molecules and is of particular significance in the field of protein NMR. Please remove after the page is dewikified. Most if not all wikilinks should simply be removed. Read more about how to correctly acknowledge RSC content.This page was imported and needs to be de-wikified.īooks should use wikilinks rather sparsely, and only to reference technical or esoteric terms that are critical to understanding the content. Permission is not required) please go to the Copyright If you want to reproduce the wholeĪrticle in a third-party commercial publication (excluding your thesis/dissertation for which If you are the author of this article, you do not need to request permission to reproduce figuresĪnd diagrams provided correct acknowledgement is given. Provided correct acknowledgement is given. If you are an author contributing to an RSC publication, you do not need to request permission Please go to the Copyright Clearance Center request page. To request permission to reproduce material from this article in a commercial publication, Provided that the correct acknowledgement is given and it is not used for commercial purposes. This article in other publications, without requesting further permission from the RSC, Luterbacher,Ĭreative Commons Attribution-NonCommercial 3.0 Unported Licence. The accuracy of this model suggests that, unlike in native lignin, ether linkages no longer appear to be randomly distributed in isolated lignin.Įstablishing lignin structure-upgradeability relationships using quantitative 1H– 13C heteronuclear single quantum coherence nuclear magnetic resonance (HSQC-NMR) spectroscopy By using a simple ether cleavage model, we were able to predict final depolymerization yields very accurately (<4% error), conclusively demonstrating the direct causal relationship between ether content and lignin activity. We then prepared a range of isolated lignin samples with a wide range of ether contents (6–46%). Here, we demonstrated that a modified HSQC-NMR method known as HSQC 0 can accurately quantify lignin functionalities in extracted lignin using several synthetic polymer models. An obstacle to the development of a conclusive causal relationship between lignin structure and upgradeability has been the difficulty to quantitatively measure lignin structural features. Past studies have suggested that lignin structural features such as ether content are correlated to lignin's upgradeability. Lignin depolymerization could provide an attractive renewable aromatic feedstock for the chemical industry.
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